Polymer models of topological insulators

Le résumé fourni par la source

Background The classic model of eukaryotic gene expression requires spatial contact between a distal enhancer and a proximal promoter. To control gene expression, various models of enhancer-blocking insulators have been proposed, including a decoy model and a topological model [1]. The decoy model suggests that the enhancer or the promoter interacts with an insulating element to prevent enhancer-promoter interactions. The topological model suggests that two or more insulating elements interact with each other to form loops. An outstanding question in the field is whether the topological model is effective at preventing enhancer-promoter interactions. Materials and methods Here we use the polymer model of chromatinized DNA and simulations of Brownian polymer dynamics to study the topological model of enhancer-blocking insulators. We consider one- and two-loop topological elements and assess spatial contacts between various regions of DNA induced or suppressed by an topological elements. Results We find that a loop formed in the region between an enhancer and a promoter in fact facilitates enhancerpromoter contacts and does not act as an enhancerblocking insulator. However, we find that sequestration of an enhancer (or promoter) within a loop is a plausible mechanism for topological insulators. Both the facilitating and insulating effects of topological elements are more dramatic with two-loop elements. Conclusions

Ce résumé expose les affirmations des auteurs. BNTIC ne l’interprète pas comme une validation indépendante des résultats.