Comparative Studies on Monolayer and Suspension Cell Cultures from a Transplantable Rat Mammary Carcinoma Containing C-Type Virus Particles

Le résumé fourni par la source

Attempts to establish monolayer cell cultures from an X-irradiation-induced transplantable rat mammary carcinoma were successful only on a short-term basis (3 to 4 passages). However, suspension cell preparations of the same tissue were successfully cultured. The tumor cells stained more darkly than normal rat cells and tended to agglutinate in the presence of commercial horse serum. Examination of the low passage monolayer cells with the electron microscope revealed the presence of C-type particles. After 151 days in vitro the suspension cells also contained C-type virus in various stages of replication

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