1067 MULTI-INSTITUTIONAL EVALUATION OF A MICRORNA EXPRESSION PROFILE DEFINING THE INVASIVE BLADDER TUMOR PHENOTYPE

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You have accessJournal of UrologyBladder Cancer: Basic Research III1 Apr 20121067 MULTI-INSTITUTIONAL EVALUATION OF A MICRORNA EXPRESSION PROFILE DEFINING THE INVASIVE BLADDER TUMOR PHENOTYPE Marc Manganiello, William Faust, Justin Zbrzezny, Christina Deliyiannis, Michelle Waknitz, Wei Huang, Jason Gee, John A. Libertino, Antonia Holway, and Kimberly Rieger-Christ Marc ManganielloMarc Manganiello Burlington, MA More articles by this author , William FaustWilliam Faust Burlington, MA More articles by this author , Justin ZbrzeznyJustin Zbrzezny Burlington, MA More articles by this author , Christina DeliyiannisChristina Deliyiannis Burlington, MA More articles by this author , Michelle WaknitzMichelle Waknitz Madison, WI More articles by this author , Wei HuangWei Huang Madison, WI More articles by this author , Jason GeeJason Gee Burlington, MA More articles by this author , John A. LibertinoJohn A. Libertino Burlington, MA More articles by this author , Antonia HolwayAntonia Holway Burlington, MA More articles by this author , and Kimberly Rieger-ChristKimberly Rieger-Christ Burlington, MA More articles by this author View All Author Informationhttps://doi.org/10.1016/j.juro.2012.02.1173AboutPDF ToolsAdd to favoritesDownload CitationsTrack CitationsPermissionsReprints ShareFacebookTwitterLinked InEmail INTRODUCTION AND OBJECTIVES MicroRNAs (miRNAs) are small, non-coding segments of regulatory RNA that have emerged as powerful biomarkers of disease severity and prognosis. We previously reported a miRNA profile capable of differentiating invasive from noninvasive urothelial carcinoma of the bladder (UCB) with a sensitivity of 100% and a specificity of 96%. The goal of this project is to validate this profile with an expanded sample pool that includes tissues from an independent institution. METHODS MiRNA expression levels in tumor tissue and cell lines were quantified by qRT-PCR. Fifty UCB cell lines and 156 UCB tumors (76 noninvasive and 80 invasive) were evaluated. Downstream targets were assessed via Western blot analysis. RESULTS On multi-institutional analysis, the original miRNA panel remained capable of distinguishing between invasive and non-invasive UCB, however, the sensitivity and specificity were both reduced to 82%. To address this we identified additional miRNAs that were correlated with invasive potential in a screen of 50 cell lines. When evaluated in tumor samples from both institutions, several of these additional miRNAs were significantly different between invasive and non-invasive tumors. A new classifier was created and tested on 156 tumors from both institutions. CONCLUSIONS Multi-institutional analysis of our panel of miRNAs capable of defining the invasive bladder tumor phenotype was confirmed albeit with a slightly reduced sensitivity and specificity. Expansion of miRNA analysis in UCB cell lines resulted in the identification of additional miRNAs, several of which were found to significantly discriminate invasive from non-invasive tumors. © 2012 by American Urological Association Education and Research, Inc.FiguresReferencesRelatedDetails Volume 187 Issue 4S April 2012 Page: e433 Advertisement Copyright & Permissions© 2012 by American Urological Association Education and Research, Inc.Metrics Author Information Marc Manganiello Burlington, MA More articles by this author William Faust Burlington, MA More articles by this author Justin Zbrzezny Burlington, MA More articles by this author Christina Deliyiannis Burlington, MA More articles by this author Michelle Waknitz Madison, WI More articles by this author Wei Huang Madison, WI More articles by this author Jason Gee Burlington, MA More articles by this author John A. Libertino Burlington, MA More articles by this author Antonia Holway Burlington, MA More articles by this author Kimberly Rieger-Christ Burlington, MA More articles by this author Expand All Advertisement Advertisement PDF downloadLoading ...

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