Effect of Terminal (Dry) Heat Treatment on Non‐Enveloped Viruses in Coagulation Factor Concentrates
Rattachement africain : gb. Niveau de preuve : code pays fourni par la source.
Le résumé fourni par la source
Terminal dry heat treatment effectively inactivated hepatitis A virus (HAV) and canine parvovirus added to high-purity factor VIII. After 24 h at 80 degrees C, HAV infectivity was reduced by > or = 4.3 log10 TCID50, as measured in a newly developed infectivity assay. The same reduction in virus titer was achieved after 2 h and before 6 h at 90 degrees C. Inactivation of hepatitis A virus was also seen in the freeze-drying step prior to heat treatment with an approximately 2.0 log10 reduction in titer. Similar results were obtained with a high-purity factor IX concentrate. Canine parvovirus was also inactivated at both temperatures, with residual infectivity being undetected after 48 h at 80 degrees C or 10 h at 90 degrees C. Canine parvovirus was not affected by lyophilisation. Canine parvovirus measurements by PCR did not reflect the levels of infectivity measured by the tissue-culture-based method. The addition of the terminal dry heat treatment to solvent/detergent could effectively eliminate the potential contamination of solvent/detergent-treated coagulation factor concentrates by non-lipid-enveloped viruses. However, careful evaluation for any increased induction of non-antigens for factor VIII, as a consequence of such treatment, is needed before use in patients can be recommended.
Ce résumé expose les affirmations des auteurs. BNTIC ne l’interprète pas comme une validation indépendante des résultats.
Le contrôle bibliographique ouvert
DOI retrouvé dans Crossref DOI retrouvé ; titre concordant.
- Titre Crossref
- Effect of Terminal (Dry) Heat Treatment on Non‐Enveloped Viruses in Coagulation Factor Concentrates
- Date Crossref
- 01/11/1994
- Éditeur
- Wiley
- Type
- journal-article
Ce recoupement confirme des métadonnées liées au DOI. Il ne confirme ni la méthode ni les conclusions de l’étude, et il ne compte pas comme une seconde source scientifique indépendante.
Où se fait cette recherche
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Scottish National Blood Transfusion Service pays non établi dans la noticeOrganisation à but non lucratif
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National Institute for Biological Standards and Control pays non établi dans la noticeStructure de recherche
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Edinburgh Royal Infirmary pays non établi dans la noticeÉtablissement de santé
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Protein Fractionation Centre pays non établi dans la noticeInstitution
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National Science Laboratory Scottish Blood Transfusion Service pays non établi dans la noticeStructure de recherche
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Edinburgh and South East Scotland Blood Transfusion Service Royal Infirmary of Edinburgh pays non établi dans la noticeInstitution
Scottish National Blood Transfusion Service, National Institute for Biological Standards and Control et Edinburgh Royal Infirmary, avec 3 autres affiliations.
Une affiliation ne permet pas de déduire la nationalité d’un auteur.