Effects of perioperative hypothermia and reactive oxygen species in the healing of colonic anastomosis in rats
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Le résumé fourni par la source
PURPOSE: We evaluated the hypothesis that induced perioperative hypothermia (32 ± 1ºC) affects the redox balance in the tissue of colonic anastomosis in rats by modifying biochemical enzymatic and non-enzymatic markers related to oxidative stress. METHODS: Forty-eight male Wistar rats were randomly divided into eight experimental groups of six animals each and underwent laparotomy, sigmoid section and immediate anastomosis. Four groups were operated under normothermia (36 ± 1ºC), and the other four under hypothermia (32 ± 1ºC). The animals were reoperated on days 3, 7 and 14 postoperatively, and two groups underwent SHAM at 3 days. From the scar tissue samples, the activity of the antioxidant enzymes superoxide dismutase (SOD) and catalase (CAT) was evaluated, and the levels of non-enzymatic markers of oxidative stress, such as reduced glutathione (GSH) and lipid peroxidation, were measured by the thiobarbituric acid reactive substances (TBARS) assay. The means were compared between groups corresponding to each day of sampling and euthanasia. RESULTS: The hypothermic groups showed a significant reduction on the activity of SOD on day 7 postoperatively, on the activity of CAT on days 7 and 14 postoperatively and on the levels of GSH on day 7 postoperatively. The level of lipid peroxidation was increased in the hypothermia group on day 7 postoperatively and decreased on day 14 compared with the normothermic groups. CONCLUSION: Perioperative hypothermia reduced the activity of the antioxidant enzymes catalase and superoxide dismutase, glutathione levels and increased lipid peroxidation in the scar tissue of colonic anastomoses in rats.
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Le contrôle bibliographique ouvert
DOI retrouvé dans Crossref DOI retrouvé ; titre concordant.
- Titre Crossref
- Effects of perioperative hypothermia and reactive oxygen species in the healing of colonic anastomosis in rats
- Date Crossref
- 01/11/2014
- Éditeur
- FapUNIFESP (SciELO)
- Type
- journal-article
Ce recoupement confirme des métadonnées liées au DOI. Il ne confirme ni la méthode ni les conclusions de l’étude, et il ne compte pas comme une seconde source scientifique indépendante.
Où se fait cette recherche
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Universidade Federal de Santa Catarina pays non établi dans la noticeUniversité ou école supérieure
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Universidade do Sul de Santa Catarina pays non établi dans la noticeUniversité ou école supérieure
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Polydoro Ernani de São Thiago University Hospital pays non établi dans la noticeÉtablissement de santé
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Federal University of Santa Catarina pays non établi dans la noticeUniversité ou école supérieure
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University of Southern Santa Catarina pays non établi dans la noticeUniversité ou école supérieure
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UFSC pays non établi dans la noticeInstitution
Universidade Federal de Santa Catarina, Universidade do Sul de Santa Catarina et Polydoro Ernani de São Thiago University Hospital, avec 3 autres affiliations.
Une affiliation ne permet pas de déduire la nationalité d’un auteur.