In vivostudies to highlight possible illegal treatments of rabbits with carbadox and olaquindox
Rattachement africain : it. Niveau de preuve : code pays fourni par la source.
Le résumé fourni par la source
For the treatment of rabbit dysentery and bacterial enteritis, veterinary practitioners often adopt veterinary medicinal products authorised for other food-producing species, but in some cases non-authorised drugs frequently used in the past, such as carbadox and olaquindox, might be illegally adopted. To verify the carbadox and olaquindox distribution and persistence in rabbit tissues, two independent in vivo studies were carried out. In the first study, 24 healthy rabbits received water medicated with carbadox at 100 mg l(-1) over a period 28 days, whereas in the second one, 24 healthy rabbits were administered water containing olaquindox at 100 mg l(-1). In each study rabbits were randomly assigned to four groups to be sacrificed respectively at 0, 5, 10 and 20 days from treatment withdrawal, for depletion studies. A control group of six animals was adopted for control and as a reservoir of blank tissues. Muscle and liver samples collected from each treated animal were stored at -20°C pending the analysis. Sensitive and robust liquid chromatography-tandem mass spectrometry analytical methods were set up for the parent compounds and their main metabolites quinoxaline-2-carboxylic acid, desoxycarbadox and 3-methylquinoxaline-2-carboxylic acid to verify their residual. Data collected demonstrate that the combination of liver as target matrix, quinoxaline-2-carboxylic acid and 3-methylquinoxaline-2-carboxylic acid as marker residue and enzymatic digestion is strategic to evidence carbadox and/or olaquindox illegal treatments in rabbits, even 20 days after treatment withdrawal at concentration levels higher than 0.5 µg kg(-1). This findings suggests that liver should be proposed as target matrix for official control in national monitoring plan.
Ce résumé expose les affirmations des auteurs. BNTIC ne l’interprète pas comme une validation indépendante des résultats.
Le contrôle bibliographique ouvert
DOI retrouvé dans Crossref DOI retrouvé, mais le titre doit être comparé manuellement.
- Titre Crossref
- <i>In vivo</i>studies to highlight possible illegal treatments of rabbits with carbadox and olaquindox
- Date Crossref
- 21/10/2015
- Éditeur
- Informa UK Limited
- Type
- journal-article
Ce recoupement confirme des métadonnées liées au DOI. Il ne confirme ni la méthode ni les conclusions de l’étude, et il ne compte pas comme une seconde source scientifique indépendante.
Où se fait cette recherche
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Istituto Zooprofilattico Sperimentale delle Venezie pays non établi dans la noticeÉtablissement de santé
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University of Padua pays non établi dans la noticeUniversité ou école supérieure
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Cluster in Biomedicine pays non établi dans la noticeOrganisation à but non lucratif
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a Food Safety Department - Chemistry Laboratory pays non établi dans la noticeStructure de recherche
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Food Safety Department – Chemistry Laboratory pays non établi dans la noticeStructure de recherche
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b Diagnostic Laboratory pays non établi dans la noticeStructure de recherche
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c Department of Comparative Biomedicine and Food Science pays non établi dans la noticeInstitution
Istituto Zooprofilattico Sperimentale delle Venezie, University of Padua et Cluster in Biomedicine, avec 4 autres affiliations.
Une affiliation ne permet pas de déduire la nationalité d’un auteur.