Sensitivity and Specificity of Histoplasma Antigen Detection by Enzyme Immunoassay
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Le résumé fourni par la source
The objective of this study was to evaluate the sensitivity and specificity of an antigen enzyme immunoassay (EIA) on urine samples for the diagnosis of histoplasmosis in dogs. This retrospective medical records review included canine cases with urine samples submitted for Histoplasma EIA antigen assay between 2007 and 2011 from three veterinary institutions. Cases for which urine samples were submitted for Histoplasma antigen testing were reviewed and compared to the gold standard of finding Histoplasma organisms or an alternative diagnosis on cytology or histopathology. Sensitivity, specificity, negative predictive value, positive predictive value, and the kappa coefficient and associated confidence interval were calculated for the EIA-based Histoplasma antigen assay. Sixty cases met the inclusion criteria. Seventeen cases were considered true positives based on identification of the organism, and 41 cases were considered true negatives with an alternative definitive diagnosis. Two cases were considered false negatives, and there were no false positives. Sensitivity was 89.47% and the negative predictive value was 95.35%. Specificity and the positive predictive value were both 100%. The kappa coefficient was 0.9207 (95% confidence interval, 0.8131-1). The Histoplasma antigen EIA test demonstrated high specificity and sensitivity for the diagnosis of histoplasmosis in dogs.
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Le contrôle bibliographique ouvert
DOI retrouvé dans Crossref DOI retrouvé ; titre concordant.
- Titre Crossref
- Sensitivity and Specificity of Histoplasma Antigen Detection by Enzyme Immunoassay
- Date Crossref
- 01/01/2015
- Éditeur
- American Animal Hospital Association
- Type
- journal-article
Ce recoupement confirme des métadonnées liées au DOI. Il ne confirme ni la méthode ni les conclusions de l’étude, et il ne compte pas comme une seconde source scientifique indépendante.
Où se fait cette recherche
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Oklahoma State University From the Department of Veterinary Clinical Sciences pays non établi dans la noticeUniversité ou école supérieure
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Kansas State University pays non établi dans la noticeUniversité ou école supérieure
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Texas A&M University pays non établi dans la noticeUniversité ou école supérieure
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Lauren Cunningham's present affiliation is North Houston Veterinary Specialists pays non établi dans la noticeInstitution
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College of Veterinary Medicine Department of Clinical Sciences pays non établi dans la noticeUniversité ou école supérieure
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MiraVista Diagnostics pays non établi dans la noticeInstitution
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Emily Kirsh's present affiliation is Orchard Software Corporation pays non établi dans la noticeInstitution
From the Department of Veterinary Clinical Sciences — Oklahoma State University, Kansas State University et Texas A&M University, avec 4 autres affiliations.
Une affiliation ne permet pas de déduire la nationalité d’un auteur.