Image Analysis Issues and Solutions for High‐Density Arrays

Le résumé fourni par la source

This chapter contains sections titled: Introduction Image Alignment, Grid Placement, and Spot Location Image Alignment Manual Spot Finding Automatic Spot Finding Spatial Segmentation of Signal and Background Pixels Pure Space-based Signal Segmentation Pure Intensity-based Signal Segmentation Mann-Whitney Segmentation The Method of Trimmed Measurements Integrating Spatial and Intensity Information for Signal Segmentation Data Quantification Quality Control Background Contamination Signal Contamination Position Offset Percentage of Ignored Pixels Percentage with an Open Perimeter Shape regularity Batch Automation Experimental Results Signal Estimation: Segmentation Method Signal Estimation: Quantification Method Background Estimation: Segmentation Method Background Estimation: Quantification Method Ratio Estimation: Quantification Method Quality Control Conclusion

Ce résumé expose les affirmations des auteurs. BNTIC ne l’interprète pas comme une validation indépendante des résultats.