Accès ouvert
2026
article
OpenAlex
Hemanta Sarmah, Grazia Iannello, Ryan Wantroba, Christopher Wu et autres
Genetically engineered human induced pluripotent stem cells (hiPSCs) are vital for disease modeling and drug discovery, yet generating clonal lines efficiently post-editing remains challenging. Here, we present a protocol to generate clonal hiPSC lines after gene editing using either electrostatic droplet- or …
us, it
(code pays fourni par la source)
Accès ouvert
2026
article
OpenAlex
Yuna Lee, Jung Yoon Choi, Hyun Jin Park, Bo Kyung Kim et autres
ABSTRACT Advancements in genomic profiling have significantly improved the classification and treatment strategies for acute myeloid leukemia (AML). However, widely utilized molecular diagnostic techniques, including targeted gene panels, are often insufficient for detecting complex structural variants, cryptic fusions, and poorly characterized driver …
us, kr
(code pays fourni par la source)
Accès ouvert
2025
preprint
OpenAlex
Sung-Soo Yoon, Chang Hyun Nam, Seung Mi Lee, J. Bae et autres
ABSTRACT Monozygotic twins are derived from the split of a single zygote early in embryogenesis. Although it was hypothesized that the timing of twining is overall associated with fetal membrane configuration of twins, i.e., chorionicity and amnionicity, our understanding of early embryonic …
us, kr, Éthiopie, be
(code pays fourni par la source)
Accès ouvert
2025
preprint
OpenAlex
Yohan An, Ji‐Hyun Lee, Joonoh Lim, Jeonghwan Youk et autres
Cancer genomes frequently carry apolipoprotein B mRNA editing catalytic polypeptide-like (APOBEC)-associated DNA mutations, suggesting APOBEC enzymes as innate mutagens during cancer initiation and evolution. However, the pure mutagenic impacts of the specific enzymes among this family remain unclear in human normal cell …
kr, us, gr, nl
(code pays fourni par la source)
Accès ouvert
2025
article
OpenAlex
Ziqi Zhou, Zhaoyu Lai, Rui Tang, Xinyu Chen et autres
As an emerging platform gaining significant attention from the biomedical community, multiplexed fluorescent imaging from imaging flow cytometry enables simultaneous detection of numerous biological targets within a single cell. Due to the spectral overlap, signals from one fluorophore can bleed into other …
us
(code pays fourni par la source)
Accès ouvert
2025
article
OpenAlex
Zunming Zhang, Yuxuan Zhu, Zhaoyu Lai, Xinyu Chen et autres
Predicting the properties of tissues or organisms from the genomics data is widely accepted by the medical community. Here we ask a question: can we predict the properties of each individual cell? Single-cell genomics does not work because the RNA sequencing process …
us
(code pays fourni par la source)
Accès ouvert
2025
preprint
OpenAlex
Seungbok Lee, June‐Young Koh, Joonoh Lim, Jaeso Cho et autres
Abstract This study aimed to implement genome sequencing using an automated pipeline for critically ill pediatric patients within a real-world healthcare system. Twenty patients under 36 months of age, admitted to the NICU/PICU or suspected of having rapidly progressive genetic disorders, were …
kr, us
(code pays fourni par la source)
2024
conference-abstract
OpenAlex
Rea Dabelic, Adonary Munoz, Micayla George, Lin Xia et autres
Abstract Cancer ranks as the second leading cause of death in the US. Central to cancer progression is the tumor microenvironment (TME), which significantly influences the disease's spread. Immune cell infiltration into the TME is crucial for effective immunotherapy. Traditional infiltration assessment …
us
(code pays fourni par la source)
Accès ouvert
2024
preprint
OpenAlex
Narendra Chirmule, Pradip Nair, Bela Desai, R. S. Khare et autres
The impact of COVID-19 disease on health and economy has been global, and the magnitude of devastation is unparalleled in modern history. Any potential course of action to manage this complex disease requires the systematic and efficient analysis of data that can …
in, us
(code pays fourni par la source)
Accès ouvert
2023
article
OpenAlex
Zunming Zhang, Xinyu Chen, Rui Tang, Yuxuan Zhu et autres
A primary challenge of high-throughput imaging flow cytometry (IFC) is to analyze the vast amount of imaging data, especially in applications where ground truth labels are unavailable or hard to obtain. We present an unsupervised deep embedding algorithm, the Deep Convolutional Autoencoder-based …
us
(code pays fourni par la source)
Accès ouvert
2022
article
OpenAlex
Rui Tang, Lin Xia, Bien Gutierrez, Ivan Gagne et autres
Classification and sorting of cells using image-activated cell sorting (IACS) systems can bring significant insight to biomedical sciences. Incorporating deep learning algorithms into IACS enables cell classification and isolation based on complex and human-vision uninterpretable morphological features within a heterogeneous cell population. …
us
(code pays fourni par la source)
Accès ouvert
2022
article
OpenAlex
Adonary Munoz, José M. Morachis
Single-cell selection and cloning is required for multiple bioprocessing and cell engineering workflows. Dispensing efficiency and outgrowth were optimized for multiple common suspension (CHO ES, Expi293F, and Jurkat) and adherent (MCF-7, A549, CHO-K1, and HEK293) cell lines. Single-cell sorting using a low …
us
(code pays fourni par la source)