Accès ouvert
2025
article
OpenAlex
Jiazhuo He, G Papa, Flora Azizi, Lydia Kirsche et autres
BACKGROUND & AIMS: CRISPR-Cas9 screening is a powerful tool for the in vivo discovery of cancer dependencies. The aim of this study was to perform in vivo CRISPR knockout screening for gastric tumor suppressors using gastric murine organoids in a subcutaneous as …
ch
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Accès ouvert
2025
article
OpenAlex
Massimo Saini, Francesc Castro-Giner, Agnes Hotz, Magdalena K. Sznurkowska et autres
CRISPR screens have become standard gene discovery platforms in various contexts, including cancer. Yet commonly available CRISPR-Cas9 tools are increasingly recognized as unfit for in vivo investigations in immunocompetent contexts, due to broad immunogenicity of bacterial nucleases and reporters. Here, we show …
ch
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Accès ouvert
2020
article
OpenAlex
Tobias Langenberg, Rodrigo Gallardo, Rob van der Kant, Nikolaos Louros et autres
The amyloid-like aggregation propensity present in most globular proteins is generally considered to be a secondary side effect resulting from the requirements of protein stability. Here, we demonstrate, however, that mutations in the globular and amyloid state are thermodynamically correlated rather than …
be
(code pays fourni par la source)