Wen Jin

Hederagenin Attenuates Cardiac Remodeling by Targeting Phosphodiesterase 9A

Liqian Chen, Xinghong Zhou, Yanting You, Jie Chen et autres

BACKGROUND AND AIM: New therapeutic strategies for heart failure are urgently needed. The protective effects of cyclic guanosine monophosphate (cGMP)-protein kinase G (PKG) pathway on heart have been widely reported. Despite phosphodiesterase 9A (PDE9A) inhibitors combating cardiac remodeling, clinically available drugs are …

cn, hk (code pays fourni par la source)

AI enhancing differential diagnosis of acute chronic obstructive pulmonary disease and acute heart failure

Yu Lao, Hao Ren, Zijian Ma, Yu Sun et autres

Differentiating acute exacerbation of chronic obstructive pulmonary disease (AECOPD) from acute heart failure (AHF) is clinically challenging due to overlapping symptoms, especially in resource-limited settings lacking radiological/ultrasonographic tools. This study developed an eXtreme Gradient Boosting (XGBoost) model for differential diagnosis using Database: …

cn, mo (code pays fourni par la source)

FigureS 1 from Long-Chain Fatty Acid Redistribution Induced by SLC27A2 Deficiency Facilitates Hypoxic Adaptation and Immunosuppression in Hepatocellular Carcinoma

Chu‐Li Fu, Wenyun Fu, Zuotian Huang, Xiaoyan He et autres

Figure S1. Expression profiles of SLC27A2 (A) Comprehensive evaluation of SLC27A2 mRNA levels was conducted across various human tissues using the GTEx database. (B) Comparative analysis of SLC27A2 mRNA expression in tumor versus non-tumor samples from HCC patients. (C) Profiling of Slc27a2 …

FigureS 13 from Long-Chain Fatty Acid Redistribution Induced by SLC27A2 Deficiency Facilitates Hypoxic Adaptation and Immunosuppression in Hepatocellular Carcinoma

Chu‐Li Fu, Wenyun Fu, Zuotian Huang, Xiaoyan He et autres

Figure S13. Associations between SLC27A2 expression and various immune markers and cell proportions in the context of Figure 5

FigureS 2 from Long-Chain Fatty Acid Redistribution Induced by SLC27A2 Deficiency Facilitates Hypoxic Adaptation and Immunosuppression in Hepatocellular Carcinoma

Chu‐Li Fu, Wenyun Fu, Zuotian Huang, Xiaoyan He et autres

Figure S2. Hypoxia-induced transcriptional repression of SLC27A2 via HNF4A inhibition (A) Analysis of DNA copy number variations in SLC genes associated with HCC, using TCGA-LIHC data. (B) Assessment of methylation levels in the SLC27A2 promoter region in tumor versus non-tumor samples, derived …

Data from Long-Chain Fatty Acid Redistribution Induced by SLC27A2 Deficiency Facilitates Hypoxic Adaptation and Immunosuppression in Hepatocellular Carcinoma

Chu‐Li Fu, Wenyun Fu, Zuotian Huang, Xiaoyan He et autres

Abstract Hypoxia, immune evasion, and metabolic reprogramming are key features of hepatocellular carcinoma (HCC) that limit the efficacy of therapies. Members of the solute carrier (SLC) family regulate metabolite homeostasis within tumor cells to maintain tumor survival under stressed conditions like hypoxia. …

FigureS 8 from Long-Chain Fatty Acid Redistribution Induced by SLC27A2 Deficiency Facilitates Hypoxic Adaptation and Immunosuppression in Hepatocellular Carcinoma

Chu‐Li Fu, Wenyun Fu, Zuotian Huang, Xiaoyan He et autres

Figure S8. Increased sensitivity of low SLC27A2-expressing HCC to glutaminase and fatty acid synthase inhibitors (A) Mechanistic illustration of FA metabolic vulnerability in SLC27A2^low HCC, reliant on glutamine's reductive metabolism and de novo FA synthesis. (B) Correlation between SLC27A2 expression and BPTES …

FigureS 15 from Long-Chain Fatty Acid Redistribution Induced by SLC27A2 Deficiency Facilitates Hypoxic Adaptation and Immunosuppression in Hepatocellular Carcinoma

Chu‐Li Fu, Wenyun Fu, Zuotian Huang, Xiaoyan He et autres

Figure S15 Low SLC27A2-induced SPP1⁺ macrophages foster an immunosuppressive microenvironment and potentially enhance responsiveness to anti-PD1 therapy (A) Analysis of Ki67 by immunofluorescence for Figure5I (B) Analysis of CD68, SPP1, CD8, and PD-1 expression by immunofluorescence. *P < 0.05, **P < 0.01, …

FigureS 4 from Long-Chain Fatty Acid Redistribution Induced by SLC27A2 Deficiency Facilitates Hypoxic Adaptation and Immunosuppression in Hepatocellular Carcinoma

Chu‐Li Fu, Wenyun Fu, Zuotian Huang, Xiaoyan He et autres

Figure S4. SLC27A2 deficiency specifically reduces LCFA uptake (A) Assessment of lipid accumulation changes following SLC27A2 modulation using triglyceride quantification. (B-D) FFA quantification was used to demonstrated that SLC27A2 specifically influences the uptake of LCFAs. Statistical significance was determined using Student’s t-test …

FigureS 6 from Long-Chain Fatty Acid Redistribution Induced by SLC27A2 Deficiency Facilitates Hypoxic Adaptation and Immunosuppression in Hepatocellular Carcinoma

Chu‐Li Fu, Wenyun Fu, Zuotian Huang, Xiaoyan He et autres

Figure S6. Energy output and lipid peroxidation influenced by fatty acid chain lengths (A-B) Effects of palmitic acid concentrations on ATP production in HCC cells under normoxia (A) and hypoxia (B). (C-D) Glucose supplementation effects on ATP output under normoxia (C), comparing …

Supplementary Data from Long-Chain Fatty Acid Redistribution Induced by SLC27A2 Deficiency Facilitates Hypoxic Adaptation and Immunosuppression in Hepatocellular Carcinoma

Chu‐Li Fu, Wenyun Fu, Zuotian Huang, Xiaoyan He et autres

supplemental methods and materials

FigureS 11 from Long-Chain Fatty Acid Redistribution Induced by SLC27A2 Deficiency Facilitates Hypoxic Adaptation and Immunosuppression in Hepatocellular Carcinoma

Chu‐Li Fu, Wenyun Fu, Zuotian Huang, Xiaoyan He et autres

Figure S11. Associations between SLC27A2 expression and various immune markers including PD-1, CTLA4, and TIM3