Paolo Cifani

Super-Resolution 3D Imaging Reveals Disarray of Dyadic Calcium Ion Channels in Failing Hearts Expressing Low Thyroid Hormone Function

Atieh Ashkezari, Megha Schmalzle, Amanda Charest, Sanketh kumar et autres

Ventricular remodeling occurring in heart failure (HF) involves structural disarray of the sarcolemma T-tubule (TT)–sarcoplasmic reticulum (SR) dyad junctions, thereby disrupting the close apposition of L-type Ca2+ channels (CaV1.2) with ryanodine receptors (RyR2) that trigger SR Ca2+ release and myofilament contraction. In …

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Supplementary Table S1 from MARK2/MARK3 Kinases Are Catalytic Codependencies of YAP/TAZ in Human Cancer

Olaf Klingbeil, Damianos S. Skopelitis, Claudia Tonelli, Toyoki Yoshimoto et autres

Paralog CRISPR screening data. Table for sgRNA sequences and raw sequencing counts of paralog CRISPR screens in 22 cancer cell lines.

Supplementary Figure S9 from MARK2/MARK3 Kinases Are Catalytic Codependencies of YAP/TAZ in Human Cancer

Olaf Klingbeil, Damianos S. Skopelitis, Claudia Tonelli, Toyoki Yoshimoto et autres

A, Sanger sequencing Chromatogram of NF2 locus in MDA-MB231 cells. B, C, Rescue experiment of MARK2+3dKO in Cas9+ YAPC cells following knockout of indicated genes. Data shown are the mean ± SD of %GFP+ (normalized to day 3 after infection). n=3. P …

Supplementary Table S6 from MARK2/MARK3 Kinases Are Catalytic Codependencies of YAP/TAZ in Human Cancer

Olaf Klingbeil, Damianos S. Skopelitis, Claudia Tonelli, Toyoki Yoshimoto et autres

FDR for synergy score paralog combinations. Table of FDR corresponding to synergy scores calculated using GEMINI.

Supplementary Figure S3 from MARK2/MARK3 Kinases Are Catalytic Codependencies of YAP/TAZ in Human Cancer

Olaf Klingbeil, Damianos S. Skopelitis, Claudia Tonelli, Toyoki Yoshimoto et autres

A, Mutational two-class comparison between 19 MARK2/3-dependent and 12 MARK2/3-independent human cancer cell lines (shown in B). Mutation data were obtained from the CCLE database. The effect size and P values were calculated for every mutated gene of the cell lines using …

Supplementary Table S5 from MARK2/MARK3 Kinases Are Catalytic Codependencies of YAP/TAZ in Human Cancer

Olaf Klingbeil, Damianos S. Skopelitis, Claudia Tonelli, Toyoki Yoshimoto et autres

P value for synergy score paralog combinations. Table of P value corresponding to synergy scores calculated using GEMINI.

Supplementary Figure S10 from MARK2/MARK3 Kinases Are Catalytic Codependencies of YAP/TAZ in Human Cancer

Olaf Klingbeil, Damianos S. Skopelitis, Claudia Tonelli, Toyoki Yoshimoto et autres

A, Western blot analysis of YAP localization following doxycycline (Dox) induced empty vector control, MKIWT or MKIMUT expression for 24h in YAPC cells. Nuclear (Nuc) and cytosolic (Cyto) fractionation are indicated. (Data shown are an extension of Fig. 6E). B, Western blot …

Supplementary Table S11 from MARK2/MARK3 Kinases Are Catalytic Codependencies of YAP/TAZ in Human Cancer

Olaf Klingbeil, Damianos S. Skopelitis, Claudia Tonelli, Toyoki Yoshimoto et autres

YAP:TEAD4 bound YAP/TAZ dependent enhancers. Peak location file for enhancers with YAP,TEAD4 binding, and reduction of signal upon YAP/TAZ knockout.

Supplementary Table S2 from MARK2/MARK3 Kinases Are Catalytic Codependencies of YAP/TAZ in Human Cancer

Olaf Klingbeil, Damianos S. Skopelitis, Claudia Tonelli, Toyoki Yoshimoto et autres

Paralog CRISPR screening data 2. Table of calculated gene level log2(fold-changes) of Paralog

Supplementary Figure S6 from MARK2/MARK3 Kinases Are Catalytic Codependencies of YAP/TAZ in Human Cancer

Olaf Klingbeil, Damianos S. Skopelitis, Claudia Tonelli, Toyoki Yoshimoto et autres

A-E, Western blot analysis of MARK2 specific substrates phosphorylation. Labeling as described in Fig 3D. Data are representative of two independent experiments. F, Lolli-pop illustration of MARK2-dependent phosphorylation sites on MST1/2 and MAP4K1-4,6,7 identified using mass spectrometry-based phosphoproteomics. SARAH= Sav/Rassf/Hpo domain, CNH= …

Supplementary Table S3 from MARK2/MARK3 Kinases Are Catalytic Codependencies of YAP/TAZ in Human Cancer

Olaf Klingbeil, Damianos S. Skopelitis, Claudia Tonelli, Toyoki Yoshimoto et autres

Cancer cell line model information. Table of mutations and cancer driver of cell lines used in this study.

Supplementary Figure S1 from MARK2/MARK3 Kinases Are Catalytic Codependencies of YAP/TAZ in Human Cancer

Olaf Klingbeil, Damianos S. Skopelitis, Claudia Tonelli, Toyoki Yoshimoto et autres

A,B, CRISPR screening results from 22 cancer cell lines. A, Abundance fold-change of positive controls (dgRNAs targeting essential genes n=28 paired with control) and negative controls (dgRNAs targeting non-coding regions n=54 and nontargeting dgRNAs n=97). Data are shown as mean ± SD …