Audrey Baldessari

A single-cell transcriptomic atlas of the pigtail macaque placenta in late gestation

Amanda Li, Rui Li, Hazel Huang, Hong Zhao et autres

BACKGROUND: The placenta is a complex organ with multiple immune and non-immune cell types that promote fetal tolerance and facilitate the transfer of nutrients and oxygen. The nonhuman primate (NHP) is a key experimental model for studying human pregnancy complications, in part …

us, ca, jp (code pays fourni par la source)

A single-cell transcriptomic atlas of the periventricular proliferative zone in the late gestation fetal brain in the pigtail macaque

Jake Cornelius, Megana Shivakumar, Taeyun Kim, Orlando Cervantes et autres

Background: The fetal brain undergoes rapid changes in late gestation, when waves of neurogenesis and gliogenesis shape cortical circuitry. The periventricular proliferative region and adjacent white matter are enriched in neuroprogenitor cells, newborn neurons, and interneurons, which is challenging to study in …

us, mx (code pays fourni par la source)

Influenza A Virus Infection Induces Immune Dysregulation in the Placenta and Fetus Without Vertical Transmission in Nonhuman Primates

Orlando Cervantes, Hazel Huang, Briana M Del Rosario, Hong Zhao et autres

Abstract Influenza A virus (IAV) infection during pregnancy is associated with stillbirth and preterm birth, but the degree to which IAV alters placental and fetal immunity is poorly understood. The objective of our study was to determine the immunologic impact of maternal …

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Granular cytoplasmic inclusions in astrocytes and microglial activation in the fetal brain of pigtail macaques in response to maternal viral infection

Raj P. Kapur, Andrew Vo, Amanda Li, Miranda Li et autres

The fetal origins of neuropsychiatric disorders are poorly understood but have been linked to viral or inflammatory injury of the developing brain. The fetal white matter is particularly susceptible to injury as myelination, axonal growth, and deep white matter tracts become established. …

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Figure S1 from Chimeric Antigen Receptor T Cell–Mediated Neurotoxicity in Nonhuman Primates

Agne Taraseviciute, Victor Tkachev, Rafael Ponce, Cameron J. Turtle et autres

A, Schema of the CD20 CAR construct. B, Cytolytic activity of human CD19 CAR T cells (solid lines) and mock (dashed lines) T cells against 51Cr-labeled human K562 and CD19-K562 and RM B-LCL1 and B-LCL2.

Figure S3 from Chimeric Antigen Receptor T Cell–Mediated Neurotoxicity in Nonhuman Primates

Agne Taraseviciute, Victor Tkachev, Rafael Ponce, Cameron J. Turtle et autres

Serum levels of 24 cytokines and chemokines measured before and at indicated days after adoptive GFP or CD20 CAR T cell transfer in the blood.

Figure S2 from Chimeric Antigen Receptor T Cell–Mediated Neurotoxicity in Nonhuman Primates

Agne Taraseviciute, Victor Tkachev, Rafael Ponce, Cameron J. Turtle et autres

Absolute numbers of RM EGFRt- (non-CAR) T cells before and at indicated days after adoptive CD20 CAR T cell transfer in the blood.

Table S2 from Chimeric Antigen Receptor T Cell–Mediated Neurotoxicity in Nonhuman Primates

Agne Taraseviciute, Victor Tkachev, Rafael Ponce, Cameron J. Turtle et autres

Grading system for neurotoxicity.

Figure S4 from Chimeric Antigen Receptor T Cell–Mediated Neurotoxicity in Nonhuman Primates

Agne Taraseviciute, Victor Tkachev, Rafael Ponce, Cameron J. Turtle et autres

A, Immunofluorescence images for CD20 (green), CD3 (red) and DAPI (blue) in R.304 before (pre, day -7) and following CD20 CAR T cell transfer (day 7 (d7)), in lymph node (LN) and brain (day 8 (d8)) and in normal RM brain. B, …

Table S3 from Chimeric Antigen Receptor T Cell–Mediated Neurotoxicity in Nonhuman Primates

Agne Taraseviciute, Victor Tkachev, Rafael Ponce, Cameron J. Turtle et autres

Antibodies and reagents used in Materials and Methods.

Table S1 from Chimeric Antigen Receptor T Cell–Mediated Neurotoxicity in Nonhuman Primates

Agne Taraseviciute, Victor Tkachev, Rafael Ponce, Cameron J. Turtle et autres

CD20 CAR T cell ex vivo expansion characteristics.

Figure S5 from Chimeric Antigen Receptor T Cell–Mediated Neurotoxicity in Nonhuman Primates

Agne Taraseviciute, Victor Tkachev, Rafael Ponce, Cameron J. Turtle et autres

CSF and serum levels of 21 cytokines and chemokines measured before and at indicated days after adoptive CD20 CAR T cell transfer.