Antonio Di Cristofano

Hras G12V induces follicular thyroid cancer with attenuated MAPK activation and increased latency compared to Kras G12D

Nicholas E. Bambach, Katherine Labella, Lee Ann Jolly, Diana Isabel Cruz et autres

Abstract RAS mutations are found in nearly 50% of follicular thyroid cancers (FTCs), frequently accompanied by secondary mutations in the PI3K/AKT pathway as tumors advance to more poorly differentiated states. To examine the role that oncogenic Hras plays in thyroid tumor initiation …

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TGFBR1 *6A and Risk for Colorectal Cancer

Allan M. Johansen, Julie T. Ziegler, Kojo Agyemang, Michael J. Pennison et autres

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Supplementary Figure 1 from One-Carbon Metabolism Inhibition Depletes Purines and Results in Profound and Prolonged Ewing Sarcoma Growth Suppression

Sara Zirpoli, Noah Copperman, Shrey Patel, Alexander R.W. Forrest et autres

Overall survival of EWS patients (n=44) expressing high or low levels of MTHFD1L, SHMT1, MTHFD1. Analysis was performed on dataset GSE17679 using R2: Genomics Analysis and Visualization Platform (http://r2.amc.nl).

Supplementary Figure 4 from One-Carbon Metabolism Inhibition Depletes Purines and Results in Profound and Prolonged Ewing Sarcoma Growth Suppression

Sara Zirpoli, Noah Copperman, Shrey Patel, Alexander R.W. Forrest et autres

Exogenous glycine deprivation does not alter the efficacy of 72h treatment of EWS cells with SHIN1. Cells were grown in glycine-free RPMI1640 with or without supplementation with 133µM glycine.

Figure 6 from One-Carbon Metabolism Inhibition Depletes Purines and Results in Profound and Prolonged Ewing Sarcoma Growth Suppression

Sara Zirpoli, Noah Copperman, Shrey Patel, Alexander R.W. Forrest et autres

Effects of SHIN1 and PRTX on one-carbon flux through the mitochondrial and cytoplasmic compartments. A, Schematic of one-carbon flux through the mitochondrial and cytosol compartments using [2,3,3-2H]L-serine. The 2H atoms in [2,3,3-2H]L-serine (blue circle) in the mitochondria are metabolized to generate [2H]formate. …

Figure 3 from One-Carbon Metabolism Inhibition Depletes Purines and Results in Profound and Prolonged Ewing Sarcoma Growth Suppression

Sara Zirpoli, Noah Copperman, Shrey Patel, Alexander R.W. Forrest et autres

Pharmacologic inhibition of one-carbon metabolism impairs Ewing sarcoma cell proliferation. A–C, Viability, measured using alamarBlue, of six Ewing sarcoma cell lines treated for 72 hours with increasing concentrations of SHIN1 (A), AGF347 (B), and PRTX (C). D, Colony-forming assay using five Ewing …

Supplementary Table 1 from One-Carbon Metabolism Inhibition Depletes Purines and Results in Profound and Prolonged Ewing Sarcoma Growth Suppression

Sara Zirpoli, Noah Copperman, Shrey Patel, Alexander R.W. Forrest et autres

Supplementary Table 1

Figure 5 from One-Carbon Metabolism Inhibition Depletes Purines and Results in Profound and Prolonged Ewing Sarcoma Growth Suppression

Sara Zirpoli, Noah Copperman, Shrey Patel, Alexander R.W. Forrest et autres

Progressive loss of the ability of relevant metabolites to rescue the proliferation of Ewing sarcoma cells after one-carbon metabolism inhibition. A–C, Rescue by exogenous adenine, thymidine, formate, and hypoxanthine addition of the growth inhibition induced by 72 hours of treatment of SK-ES-1 …

Figure 4 from One-Carbon Metabolism Inhibition Depletes Purines and Results in Profound and Prolonged Ewing Sarcoma Growth Suppression

Sara Zirpoli, Noah Copperman, Shrey Patel, Alexander R.W. Forrest et autres

Pharmacologic inhibition of one-carbon metabolism results in inhibition of Ewing sarcoma spheroid growth and colony formation, resulting in G1 phase to early S-phase arrest. A, Analysis of the inhibitory effect of SHIN1 and PRTX on the growth of SK-ES-1 spheroids. *, P …

Figure 7 from One-Carbon Metabolism Inhibition Depletes Purines and Results in Profound and Prolonged Ewing Sarcoma Growth Suppression

Sara Zirpoli, Noah Copperman, Shrey Patel, Alexander R.W. Forrest et autres

In vivo efficacy of genetic one-carbon metabolism inhibition. A, shRNA-mediated depletion of SHMT2 severely impairs in vivo tumor growth in SK-ES-1 xenografts, resulting in significantly smaller tumors, as shown by the difference in weight of the excised tumors at the end of …

Table 1 from One-Carbon Metabolism Inhibition Depletes Purines and Results in Profound and Prolonged Ewing Sarcoma Growth Suppression

Sara Zirpoli, Noah Copperman, Shrey Patel, Alexander R.W. Forrest et autres

EC50 of the three inhibitors in Ewing sarcoma cell lines

Supplementary Figure 3 from One-Carbon Metabolism Inhibition Depletes Purines and Results in Profound and Prolonged Ewing Sarcoma Growth Suppression

Sara Zirpoli, Noah Copperman, Shrey Patel, Alexander R.W. Forrest et autres

A) Effect of the indicated treatments on the induction of cell death as measured by LDH release. B) Effect (at day 10) of delayed hypoxanthine addition or SHIN1 removal, both at day 6, on the growth inhibition induced in SK-ES-1 and TC-71 …