Ana Zarubica

Mucosal tissue cues shape B cell memory through the IgA BCR

María Pía Holgado, Samuel Origlio, Lucas Bertoia, Myriam Moussa et autres

B cells generate plasma cells (PCs) and memory B cells (MBCs) to combat recurrent pathogens. B cell receptor (BCR) affinity dictates fate decisions in response to model antigens, but the factors regulating B cell fate during infection remain unknown. Here, we used …

fr, ch, gb, nz (code pays fourni par la source)

Plasmacytoid dendritic cells are dispensable or detrimental in murine systemic or respiratory viral infections

Clemence Ngo, Camille Pierini-Malosse, Khalissa Rahmani, Michael Valente et autres

Plasmacytoid dendritic cells (pDCs) are major producers of type I/III interferons. As interferons are crucial for antiviral defense, pDCs are assumed to play an essential role in this process; however, robust evidence supporting this dogma is scarce. Genetic or pharmacological manipulations that …

fr, se (code pays fourni par la source)

Mucosal B cell memory selection integrates tissue-specific cues via the IgA BCR

María Pía Holgado, Samuel Origlio, Lucas Bertoia, Myriam Moussa et autres

Abstract B cells engender plasma cells (PCs) and memory B cells (MBCs) to combat recurrent pathogens. While B cell receptor (BCR) affinity dictates fate decisions in clonally-restricted set-ups, the driving factors in more complex systems remain unknown. Here, we show that memory …

fr, ch, gb (code pays fourni par la source)

Intranasally administrated fusion-inhibitory lipopeptides block SARS-CoV-2 infection in mice and enable long-term protective immunity

Saïd Mougari, Valérie Favède, Camilla Predella, Olivier Reynard et autres

We have assessed antiviral activity and induction of protective immunity of fusion-inhibitory lipopeptides derived from the C-terminal heptad-repeat domain of SARS-CoV-2 spike glycoprotein in transgenic mice expressing human ACE2 (K18-hACE2). The lipopeptides block SARS-CoV-2 infection in cell lines and lung-derived organotypic cultures. …

fr, us, it (code pays fourni par la source)

879 Deep immunoprofiling of tumor mouse models for preclinical studies

Anaïs Joachim, Marielle Mello, Emilie Maturin, Lillia Hadjem et autres

Background Preclinical tumor oncology research relies on analysis of tumor cell lines implanted into immune-competent mice in case of syngeneic mouse tumors, or severely immune-deficient mouse models carrying nude, SCID IL-2Rg or Rag mutations in case of human Cell derived xenograft (CDX) …

fr (code pays fourni par la source)

Intranasal Multiepitope PD‐L1‐siRNA‐Based Nanovaccine: The Next‐Gen COVID‐19 Immunotherapy (Adv. Sci. 40/2024)

Rita C. Acúrcio, Ron Kleiner, Daniella Vaskovich‐Koubi, Bárbara Carreira et autres

Nanovaccine In article number 2404159, Helena F. Florindo, Ronit Satchi-Fainaro, and co-workers describe the development of a next-generation thermostable multi-epitope mannosylated nanovaccine administered intranasally. This innovative nanovaccine generates mucosal immunity while regulating PD-1/PD-L1 axis within the DC-T cell interface, coordinating CD4 and …

Intranasal Multiepitope PD‐L1‐siRNA‐Based Nanovaccine: The Next‐Gen COVID‐19 Immunotherapy

Rita C. Acúrcio, Ron Kleiner, Daniella Vaskovich‐Koubi, Bárbara Carreira et autres

The first approved vaccines for human use against severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) are nanotechnology-based. Although they are modular, rapidly produced, and can reduce disease severity, the currently available vaccines are restricted in preventing infection, stressing the global demand for …

pt, il, fr (code pays fourni par la source)

Improving laboratory animal genetic reporting: LAG-R guidelines

Lydia Teboul, James Amos‐Landgraf, Fernando Benavides, Marie‐Christine Birling et autres

The biomedical research community addresses reproducibility challenges in animal studies through standardized nomenclature, improved experimental design, transparent reporting, data sharing, and centralized repositories. The ARRIVE guidelines outline documentation standards for laboratory animals in experiments, but genetic information is often incomplete. To remedy …

gb, us, fr, tw, uy, au, dk, cn, il, de, es, kr, ca, cz, jp, ch (code pays fourni par la source)

Plasmacytoid dendritic cells are dispensable or detrimental in murine systemic or respiratory viral infections

Clemence Ngo, Camille Pierini-Malosse, Khalissa Rahmani, Michael Valente et autres

Abstract Plasmacytoid dendritic cells (pDCs) are major producers of type I/III interferons. As interferons are crucial for antiviral defense, pDCs are assumed to play an essential role in this process. However, robust evidence supporting this dogma is scarce. Genetic or pharmacological manipulations …

fr, cn, us, la, se, ca (code pays fourni par la source)

Abstract 86: Deep immuno-profiling of syngeneic tumor mouse models for preclinical studies

Anaïs Joachim, Emilie Maturin, Marielle Mello, Lilia Hadjem et autres

Abstract The identification of the major cellular players involved in the progression of a type of cancer is a key step for the success of new immunotherapies for personalized medicine. Immune cells play critical functions in cancer, and mice with intact immune …

fr (code pays fourni par la source)

Abstract 85: Immunomics of immune-deficient mouse models before and after CD34 humanization

Hervé Luche, Lilia Hadjem, Marielle Mello, Priscilla Canavese et autres

Abstract Preclinical tumor oncology research relies historically on the analysis of mouse or human tumor cell lines implanted onto severely immune-deficient mouse models carrying nude, SCID or Rag mutations on different genetical background (C57Bl/6N, BalbC, NMRI, CB17 or NOD). These lines harbor …

fr (code pays fourni par la source)

Neutralization of SARS-CoV-2 and Intranasal Protection of Mice with a nanoCLAMP Antibody Mimetic

Quentin Pagneux, Nathalie Garnier, Manon Fabregue, Sarah Sharkaoui et autres

Intranasal treatment, combined with vaccination, has the potential to slow mutational evolution of viruses by reducing transmission and replication. Here, we illustrate the development of a SARS-CoV-2 receptor-binding domain (RBD) nanoCLAMP and demonstrate its potential as an intranasally administered therapeutic. A multi-epitope …

fr, us (code pays fourni par la source)