Takuya Yashiro

Cyclin-dependent kinase (CDK) 8 and its paralog CDK19 develop group 2 innate lymphoid cell–related lung fibrosis by activating STAT5

Masaya Matsuda, Yuna Fujiwara, Emi Ishizu, Hayato Shimora et autres

Group 2 innate lymphoid cells (ILC2s) produce large amounts of IL-5, IL-13, and amphiregulin, which are involved in the development of lung fibrosis. Activation of ILC2s is mediated by phosphorylation of STAT5. Although STAT5 has tyrosine and serine phosphorylation sites, the mechanisms …

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Raman flow cytometry using time-delay integration

Matthew R. Lindley, Toshiki Kubo, Stéphanie Devineau, Menglu Li et autres

Raman flow cytometry offers chemically sensitive, label-free measurement of cells and particles; however, the technique suffers from low cell throughput due to the weak Raman signal. Here, we demonstrate the use of time-delay integration (TDI) to achieve Raman flow cytometry combined with …

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Immunostimulatory effects of Heyndrickxia coagulans SANK70258

Yuki Ikeda, Niya Yamashita, Naoto Ito, Natsuki Minamikawa et autres

Here, we examined the immunomodulating effects of Heyndrickxia coagulans SANK70258 (SANK70258). Mouse splenocytes treated with γ-ray-irradiated SANK70258 produced higher levels of interferon (IFN)-γ than those with 7 types of lactic acid bacteria. IFN-γ was mainly produced by natural killer (NK) cells, involving …

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Kaempferol Exerts Anti‐Inflammatory Effects by Accelerating Treg Development via Aryl Hydrocarbon Receptor‐Mediated and PU.1/IRF4‐Dependent Transactivation of the Aldh1a2 /Raldh2 Gene in Dendritic Cells

Miki Takahashi, Kazuki Nagata, Yumi Watanuki, Masaki Yamaguchi et autres

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Raman flow cytometry using time delay integration

Matthew R. Lindley, Toshiki Kubo, Stéphanie Devineau, Menglu Li et autres

Raman flow cytometry offers chemically sensitive, label-free measurement of cells and particles; however, the technique suffers from low cell throughput due to the weak Raman signal. Here, we demonstrate the use of time-delay integration to achieve Raman flow cytometry combined with dual-sided …

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The powerful potential of amino acid menthyl esters for anti‐inflammatory and anti‐obesity therapies

Seidai Takasawa, Kosuke Kimura, M Miyanaga, Takuya Uemura et autres

Our newly developed menthyl esters of valine and isoleucine exhibit anti-inflammatory properties beyond those of the well-known menthol in macrophages stimulated by lipopolysaccharide (LPS) and in a mouse model of colitis induced by sodium dextran sulfate. Unlike menthol, which acts primarily through …

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The gut lactic acid bacteria metabolite, 10-oxo-cis-6,trans-11-octadecadienoic acid, suppresses inflammatory bowel disease in mice by modulating the NRF2 pathway and GPCR-signaling

Miki Ando, Kazuki Nagata, Ryuki Takeshita, Naoto Ito et autres

Various gut bacteria, including Lactobacillus plantarum , possess several enzymes that produce hydroxy fatty acids (FAs), oxo FAs, conjugated FAs, and partially saturated FAs from polyunsaturated FAs as secondary metabolites. Among these derivatives, we identified 10-oxo- cis -6, trans -11-octadecadienoic acid (γKetoC), …

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Kaempferol exerts anti-inflammatory effects by accelerating Treg development via AhR-mediated and PU.1/IRF4-dependent transactivation of the Aldh1a2/ RALDH2 gene in dendritic cells

Miki Takahashi, Kazuki Nagata, Yumi Watanuki, Masaki Yamaguchi et autres

Abstract Retinaldehyde dehydrogenase 2 encoded in the Aldh1a2 gene (RALDH2) is expressed in intestinal dendritic cells (DCs) in mesenteric lymph nodes (MLNs) and plays a crucial role in Treg development by producing retinoic acid. In the present study, we screened food ingredients …

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NR4A3 deficiency ameliorates contact hypersensitivity and exacerbates psoriasis by regulating gene expression in dendritic cells

Mayuka Katagiri, Naoto Ito, Kazuki Nagata, Nakano Shiori et autres

Abstract NR4A3 is a transcription factor that belongs to the nuclear receptor superfamily. To reveal the roles of NR4A3 in skin diseases, we constructed contact hypersensitivity (CHS)- and imiquimod-induced psoriasis models in Nr4a3 -/- mice. In the CHS induced Nr4a3 -/- mice, …

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Rose flavor compound b-damascone regulates dendritic cell-mediated immunoresponses by modulating the NRF2 pathway and ameliorates contact hypersensitivity

Naoki Kodama, Hikaru Okada, Kazuki Nagata, Miki Ando et autres

Background: Numerous pharmacologically beneficial compounds have been isolated from natural products derived from plants; these compounds are often characterized as phytochemicals and are used in flavors, spices, fragrances, and colors. In the current study, we aimed to obtain novel immunomodulators from aroma …

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Butyrate, Valerate, and Niacin Ameliorate Anaphylaxis by Suppressing IgE-Dependent Mast Cell Activation: Roles of GPR109A, PGE2, and Epigenetic Regulation

Kazuki Nagata, Daisuke Ando, Tsubasa Ashikari, Kandai Ito et autres

Short-chain fatty acids (SCFAs) are produced by the intestinal microbiota during the fermentation of dietary fibers as secondary metabolites. Several recent studies reported that SCFAs modulate the development and function of immune-related cells. However, the molecular mechanisms by which SCFAs regulate mast …

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Kaempferol Suppresses the Activation of Mast Cells by Modulating the Expression of FcεRI and SHIP1

Kazuki Nagata, Sanae Araumi, Daisuke Ando, Naoto Ito et autres

In the present study, we evaluated the effects of kaempferol on bone marrow-derived mast cells (BMMCs). Kaempferol treatment significantly and dose-dependently inhibited IgE-induced degranulation, and cytokine production of BMMCs under the condition that cell viability was maintained. Kaempferol downregulated the surface expression …

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